Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification – Introduction of a polynucleotide molecule into or...
Reexamination Certificate
2006-09-19
2006-09-19
Ketter, James (Department: 1636)
Chemistry: molecular biology and microbiology
Process of mutation, cell fusion, or genetic modification
Introduction of a polynucleotide molecule into or...
C435S470000, C435S285200
Reexamination Certificate
active
07109034
ABSTRACT:
The invention relates to a method for permeabilization of a cell structure consisting of a single cell, an intracellular structure or an organelle comprising the following steps: (a) microelectrodes, preferably two carbon fiber electrodes or hollow fiber electrodes, are provided, (b) the microelectrodes are connected to a power supply, (c) the electrodes, individually controlled by high-graduation micromanipulators, are placed close to the cell structure at an appropriate inter-electrode distance, and (d) a highly focused electric field of a strength sufficient to obtain electroporation is applied between the electrodes. The method may be used in order to transfer cell impermeant solutes, such as drugs or genes, into the cell structure or out of the cell structure, in biosensors, in the treatment of tumours and neurodegenerative diseases and in the study of biophysical processes.
REFERENCES:
patent: 5019034 (1991-05-01), Weaver et al.
patent: 5137817 (1992-08-01), Busta et al.
patent: 5874268 (1999-02-01), Meyer
patent: 6261815 (2001-07-01), Meyer
patent: 2002/0061589 (2002-05-01), King et al.
patent: 2002/0062126 (2002-05-01), Lewis et al.
patent: 2002/0127723 (2002-09-01), Palermo
patent: 2002/0133137 (2002-09-01), Hofmann
patent: 2002/0160437 (2002-10-01), Meyer
patent: 2002/0198485 (2002-12-01), Dev et al.
patent: 2002/0198512 (2002-12-01), Seward
patent: 2003/0009113 (2003-01-01), Olson
patent: 2003/0009148 (2003-01-01), Hayakawa
patent: 2003/0017598 (2003-01-01), Burke et al.
patent: 19950133907 (1995-05-01), None
patent: 1997/05922 (1997-02-01), None
patent: WO 99/24110 (1999-05-01), None
patent: WO 00/34434 (2000-06-01), None
patent: WO 01/07583 (2001-02-01), None
patent: WO 01/48180 (2001-07-01), None
patent: WO 02/31171 (2002-04-01), None
patent: WO 2/100459 (2002-12-01), None
Needham, Measurement of interbilayer adhesion energies, 1993, Methods in Enzymology vol. 220, pp. 111-129.
Karlsson, et al., “Electroinjection of Colloid Particles and Biopolymers into Single Unilamellar Liposomes and Cells for Bioanalytical Applications”, Anal. Chem., 72, 5857-5862 (2001).
Swartz, et al., “Sparking New Frontiers: Using in Vivo Electroporation for Genetic Manipulations” Development Biology 233, 13-21 (2001).
Chiu Daniel
Eriksson Peter
Lundqvist Anders
Orwar Owe
Cellectricon AB
Conlin David G.
Edwards Angell Palmer & & Dodge LLP
Ketter James
Patton Stephana E.
LandOfFree
Method for electro-permeabilization of individual cellular... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for electro-permeabilization of individual cellular..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for electro-permeabilization of individual cellular... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3529293