Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2005-10-18
2005-10-18
Fredman, Jeffrey (Department: 1637)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
Reexamination Certificate
active
06955882
ABSTRACT:
The invention provides a method that allows the construction of a chimeric and/or modified and/or reconstructed DNA molecule from two DNA fragments in a defined order and orientation, and to clone the molecule one step in a suitable vector using site specific recombination. No initial step of classical cloning via restriction enzymes is needed, in contrast to the classical recombination systems. This method allows the reliability of the recombination method for cloning with the flexibility of PCR to introduce modifications in the insert sequence. Moreover, this method allows the construction of chimerical DNA molecules associating two different elements, such as promoter-gene association or fusion proteins.
REFERENCES:
patent: H1531 (1996-05-01), Blumentals et al.
patent: 2002/0007051 (2002-01-01), Cheo et al.
patent: 2004/0103453 (2004-05-01), Dudler et al.
MultiSite Gateway™ Three Fragment Vector Construction Kit, Instruction Manual, InvitrogenTM Catalog No. 12537-023, Version A, Jul. 31, 2002.
Gateway ™ Cloning Technology Catalog, Life Technologies®.
Droual Anne-Marie
Frankard Valérie Marie-Noëlle
Hatzfield Yves
Calamita Heather G.
CropDesign N.V.
Dilworth & Barrese
Fredman Jeffrey
Pokalsky Ann R.
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