Method for DNA amplification using DNA blocking probes

Details Method for DNA amplification using DNA blocking probes Method for DNA amplification using DNA blocking probes

2003-12-08

2008-03-18

Kim, Young J. (Department: 1637)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S091200

Reexamination Certificate

active

07344834

ABSTRACT:
A process is disclosed for the amplification of a DNA template by subjecting a sample of biological material containing a target nucleotide sequence to amplification using a non-extendable oligonucleotide blocker. The method comprises using oligonucleotide primers and blockers to create primer extension products that are susceptible to cleavage by double-strand-specific ribonucleases. The continuous production and cycling of ribonuclease cleaved products allows for amplification of a target sequence.

REFERENCES:
patent: 5645987 (1997-07-01), Richards
patent: 5783392 (1998-07-01), Seibl et al.
patent: 5916777 (1999-06-01), Kacian et al.
patent: 6251639 (2001-06-01), Kurn
patent: 6893819 (2005-05-01), Sorge

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