Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Reexamination Certificate
2005-03-22
2005-03-22
Gitomer, Ralph (Department: 1651)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
C435S029000, C435S380000, C435S383000
Reexamination Certificate
active
06869771
ABSTRACT:
A method for distributing an equal volume of attached confluent living cells in each well of a multi-well plate used in a bioassay, comprising the following steps:(a) growing attached living cells on a medium until the cells are confluent; (b) treating the attached confluent living cells with an enzyme solution, whereby the solution and the attached confluent living cells make a cell suspension; (c) filtering the cell suspension with a filter; (d) collecting a cell suspension filtrate containing uniformly distributed attached confluent living cells therein; and (e) distributing an equal volume of the cell suspension filtrate into wells of a plate whereby each well has an equal volume of cells for use in any bioassay.
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patent: 5753229 (1998-05-01), Mordoh et al.
patent: 6723699 (2004-04-01), Lewis et al.
Moller S. A Filter Immuno Plaque Assay for the Detection of Antibody Secreting Cells In Vitro. J of Immuno Methods 79(2)195-204, 1985.*
Forsyth E. Proliferation and Extracellular Matrix Production by Human Infragenicular Smooth Muscle Cells in Response to Interleukin-1beta. J of Vascular Surgery 26(6)1002-8, Dec. 1997.
Access Business Group International LLC
Alticor Inc.
Gitomer Ralph
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