Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1990-08-31
1993-11-23
Zitomer, Stephanie W.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 15, 435 18, 435 912, C12Q 168, C12Q 148, C12Q 134, C12P 1934
Patent
active
052643433
ABSTRACT:
A method of electing the presence or absence of exposed nuclear DNA is described. Cells are reacted with a reaction composition comprising DNA polymerase I, DNase I, and the nucleotides dATP, dCTP, dGTP, and dTTP or dUTP, at least one of said nucleotides being biotin labeled. Biotin labeled nucleotides incorporated in exposed DNA are detected. Also described is a kit useful for detecting the presence or absence of exposed DNA in cells.
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Krystosek Alphonse
Puck Theodore T.
Eleanor Roosevelt Institute
Zitomer Stephanie W.
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