Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2006-10-03
2006-10-03
Horlick, Kenneth R. (Department: 1637)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S091100, C435S091200
Reexamination Certificate
active
07115376
ABSTRACT:
Method and composition for detecting one or more selected polynucleotide regions in a target polynucleotide. In one embodiment of the invention, a plurality of different-sequence probe pairs are added to a target polynucleotide, where each probe pair includes two polynucleotide probe elements which are complementary in sequence to adjacent portions of a selected one of the target sequences in the target polynucleotide. In each probe pair, one of the probe elements contains a non-polynucleotide polymer chain which imparts a distinctive mobility to the associated probe pair, when the elements in the pair are ligated. The other element in the pair contains a detectable reporter label. After the probe pairs have been allowed to hybridize with the target polynucleotide, the hybridized polynucleotides are treated under conditions effective to ligate the end subunits of target-bound probe elements when their end subunits are base-paired with adjacent target bases. The ligated probe pairs are then released from the target polynucleotide and separated electrophoretically in a sieving matrix, or chromatographically.
REFERENCES:
patent: 4683202 (1987-07-01), Mullis
patent: 4879214 (1989-11-01), Kornher et al.
patent: 4883750 (1989-11-01), Whiteley et al.
patent: 4925785 (1990-05-01), Wang et al.
patent: 5011769 (1991-04-01), Duck et al.
patent: 5061361 (1991-10-01), Gordon
patent: 5093232 (1992-03-01), Urdea et al.
patent: 5108568 (1992-04-01), Van Alstine
patent: 5171534 (1992-12-01), Smith et al.
patent: 5470705 (1995-11-01), Grossman et al.
patent: 5514543 (1996-05-01), Grossman et al.
patent: 5580732 (1996-12-01), Grossman et al.
patent: 5777096 (1998-07-01), Grossman et al.
patent: 5807682 (1998-09-01), Grossman et al.
patent: 6759202 (2004-07-01), Grossman et al.
patent: WO 92/08728 (1992-05-01), None
Agrawal, S. et al., “Site-Specific Functionalization of Oligodeoxynucleotides for Non-Radioactive Labeling,”Tetrahedron Letters, 31(11):1543-1546 (1990).
Barany, F., “Genetic Disease Detection and DNA Amplification Using Cloned Thermostable Ligase,”Proc. Natl. Acad. Sci. USA, 88:189-193 (1991).
Chen et al., “Low-Cost, High-Sensitivity Laser-Induced Fluorescence Detection for DNA Sequencing by Capillary Gel Electrophoresis,”J. Chrom.,559:237-246 (1991).
Cload, S. et al., “Polyether Tethered Oligonucleotide Probes,”J. Am. Chem. Soc.,113: 6324 (1991).
Cohen, A. et al., “High-Performance Capillary Electrophoretic Separation of Bases, Nucleosides, and Oligonucleotides: Retention Manipulation via Micellar Solutions and Metal Additives,”Anal. Chem.,59(7): 1021-1027 (1987).
Cunico, R. et al., “Characterization of Polyethylene Glycol Modified Proteins Using Charge Reversed Capillary Electrophoresis,”J. Chrom.,559:467-477 (1991).
Duck, P. et al., “Probe Amplifier System Based on Chimeric Cycling Oligonucleotides,”BioTechniques, 9(2):142-148 (1990).
Haralambidis, J. et al., “The Synthesis of Polyamide-Oligonucleotide Conjugate Molecules,”Nuc. Acids Res.,18(3):493-499 (1990).
Jorgenson, J. et al., “Capillary Zone Electrophoresis,”Science, 222:266-272 (1983).
Kambara et al., “Real Time Automated Simultaneous Double-Stranded DNA Sequencing Using Two-Color Fluorophore Labeling,”BioTechnology, 9:648-651 (1991).
Karger et al., “Multiwavelength Fluorescence Detection for DNA Sequencing Using Capillary Electrophoresis,”Nuc. Acids Res.,19(18):4955-4962 (1991).
Kornher, J., “Mutation Detection Using Nucleotide Analogs that Alter Electrophoretic Mobility,”Nuc. Acids Res.,17(19): 7779-7784 (1989).
Landegren, U. et al., “A Ligase-Mediated Gene Detection Technique,”Science, 241:1077-1080 (1988).
Livak, K. et al., “Detection of Single Base Differences Using Biotinylated Nucleotides with Very Long Linker Arms,”Nuc. Acids Res., 20(18):4831-4837 (1992).
Mayer, P. et al., “Theory of DNA Sequencing Using Free Solution Electrophoresis of Protein-DNA Complexes,”Analytical Chem., 66:1777-1780 (1994).
Müller, W. et al., “Polyethylene Glycol Derivatives of Base and Sequence DNA Ligands: DNA Interaction and Application for Base Specific Separation of DNA Fragments by Gel Electrophoresis,”Nuc. Acids Res.,9(1):95-119 (1981).
Nathakarkitkool, S. et al., “High-Resolution Capillary Electrophoretic Analysis of DNA in Free Solution,”Electrophoresis, 13:18-31 (1992).
Noolandi, J., “A New Concept for Sequencing DNA by Capillary Electrophoresis,”Electrophoresis, 13: 394-395 (1992).
Prober, J. et al., “A System for Rapid DNA Sequencing with Fluorescent Chain-Terminating Dideoxynucleotides,”Science, 238:336-341 (1987).
Righetti, P., “Recent Developments in Electrophoretic Methods,”J. Chrom., 516:3-22 (1990).
Saiki, R. et al., “Enzymatic Amplification of β-Globin Genomic Sequences and Restriction Site Analysis for Diagnosis of Sickle-Cell Anemia,”Science, 230:1350-1354 (1985).
Skolnick, M. et al., “Simultaneous Analysis of Mutliple Polymorphic Loci Using Amplified Sequence Polymorphisms (ASPs),”Genomics, 2: 273-279 (1988).
Winn-Deen, E. et al., “Sensitive Fluorescence Method for Detecting DNA Ligation Amplification Products,”Clinical Chemistry, 37(9): 1522-1523 (1991).
Wu, D. et al., “Specificity of the Nick-Closing Activity of Bacteriophage T4 DNA Ligase,”Gene, 76: 245-254 (1989).
Wu, D. et al., “The Ligation Amplification Reaction (LAR)-Amplification of Specific DNA Sequences Using Sequential Rounds of Template-Dependent Ligation,”Genomics, 4:560-569 (1989).
“Optimization and Troubleshooting DNA Sequencing with the Model 370A for use with Version 1.20 data analysis software,”Applied Biosystems User Bulletin, 7:1-15 (Apr. 15, 1998).
Fung Steven
Grossman Paul David
Menchen Steven Michael
Winn-Deen Emily Susan
Woo Sam Lee
Applera Corporation
Horlick Kenneth R.
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