Chemistry: analytical and immunological testing – Nuclear magnetic resonance – electron spin resonance or other...
Reexamination Certificate
2007-07-31
2007-07-31
Gakh, Yelena G. (Department: 1743)
Chemistry: analytical and immunological testing
Nuclear magnetic resonance, electron spin resonance or other...
C436S015000
Reexamination Certificate
active
10359576
ABSTRACT:
Disclosed is a method for distinguishing between protein variants using high field asymmetric waveform ion mobility spectrometry (FAIMS). A method according to the instant invention includes the steps of providing a FAIMS analyzer region defined by a space between two spaced-apart electrodes, and providing predetermined conditions within said FAIMS analyzer region. Ions of an analyte protein are produced from a solution having predetermined properties and including the analyte protein, using a suitable ionization source. The ions are introduced into the FAIMS analyzer region, transmitted therethrough, and detected. An intensity of the detected ions is measured at a predetermined CV value. At least a difference is determined, between the detected intensity and a reference value, the reference value relating to an expected intensity at the predetermined CV value for a reference form of the analyte protein. The analyte protein is registered as a variant of the reference form, in dependence upon the determined differences.
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Guevremont Roger
Purves Randy
Freedman & Associates
Gakh Yelena G.
Thermo Finnigan LLC
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