Method for disrupting genes using tobacco retrotransposon

Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or...

Reexamination Certificate

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C800S298000, C800S306000, C435S069100, C435S468000, C435S430000

Reexamination Certificate

active

09979430

ABSTRACT:
The present invention relates to a method for disrupting a gene in a plant using a tobacco retrotransposon, comprising the steps of introducing the retrotransposon into the plant, and culturing and regenerating the plant, into which the retrotransposon is introduced, to produce a transformed plant.

REFERENCES:
Casacuberta et al. EMBO J. 14:2670-2678 (1995).
Hirochika et al The EMBO Journal, vol. 12 (6), pp. 2521-2528 (1993).
Hirochika et al (PNAS (1996) 93:7783-7788.
Feuerbach, F. et al., “Retrovirus-Like End Processing of the Tobacco Tnt1 Retrotransposon Linear Intermediates of Replication”,Journal of Virology71(5):4005-4015, 1997.
Results from a summarized group of a special research area, based on molecular variable plant organ plan “A Plan Concerning Plant Organs”, Research Result Proceedings of 1995 (1995), p. 114-115 (English abstract translation).
Hirochika et al.,The Plant Cell, 8:725-734 (1996).

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