Method for disinfecting and washing of immobilized lactase

Details Method for disinfecting and washing of immobilized lactase Method for disinfecting and washing of immobilized lactase

1981-10-01

1984-08-14

Naff, David M.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

426 41, 426 42, 435180, 435207, 435260, 435800, C12P 1902, C12N 1108, C12N 938, A23C 2102

Patent

active

044657722

ABSTRACT:
Effective disinfection and washing of immobilized lactase from Aspergillus oryzae is carried out after a period of hydrolyzing lactose by treatment of the immobilized lactase with aqueous solutions of pH's 2 to 4 and 6 to 8.7, one or both of which contain a disinfectant. The solution having a pH of 6 to 8.7 may be a phosphate buffered solution. The lactase may be immobilized on a high polymer compound by covalent linkage. The immobilized lactase has outstanding resistance to chemicals and wide pH stability so that decrease of lactase activity is minimized.

REFERENCES:
patent: 4239854 (1980-12-01), Hirohara et al.
Olson et al., Immobilized Enzymes in Food and Microbial Processes, Plenum Press, NY, 1974, (pp. 56-61).
Weetall et al., Preparation of Immobilized Lactase, Biotech. & Bioeng., vol. XVI, 1974, (pp. 689-696).
Pitcher, Jr. et al., The Preparation, Characterization and Scale-up of a Lactase System Immobilized to Inorganic Supports for the Hydrolysis of Acid Whey, Methods in Enzymology, vol. XLIV, Academic Press, NY, 1976, (pp. 792-809).
Pitcher, Jr. et al., Development of an Adsorbed Lactase Immobilized Enzyme System, Enzyme Engineering, vol. 3, Plenum Publishing Corp., 1978, (pp. 483-496).
Charles et al., Enzymes Immobilized on Alumina and Stainless Steel Supports, Biotech & Bioeng., vol. XVII, 1975, (pp. 203-210).
Jour. of Food Sci., vol. 42, No. 2, (1975), pp. 291-296.
Chem. Absts., vol. 90, No. 9, (Feb. 26, 1979), p. 389, Abstract 70574x.

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