Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1988-05-03
1992-06-16
Kepplinger, Esther L.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 21, 435 28, 435 29, 435 72, 435 721, 435 75, 435 79, 436 63, 436166, 436172, 436519, 436521, 436532, 436548, 436805, 436825, G01N 3353
Patent
active
051224534
ABSTRACT:
A flow cytometry method for reproducibly detecting and counting a lymphocyte population of interest in a leukocyte suspension or whole blood sample in which the red cells are subsequently lysed. The suspension (or sample) is combined with a reagent comprising a primary antibody, either native, carrying an attached enzyme or biotin or other label, and a fixative reagent, in either order. Where the enzyme is not attached, an enzyme is coupled specifically to the primary antibody. The fixed suspension is reacted with a color-producing enzyme-cytochemical reagent. The suspension, now including stained and unstained fixed cells, is passed through a flow cytometer and the cells are characterized and counted on the basis of their light-scattering and light-absorbing properties.
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Ansley Hudson R.
Kanter Robert J.
Kim Young R.
Martin Grace E.
Ornstein Leonard
Greenman Jeffrey M.
Kepplinger Esther L.
Spiegel Carol A.
Technicon Instruments Corporation
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