Method for discriminating between unstained and absorbing dye st

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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2504612, 356 39, 424 3, 436 63, 436519, 436805, G01N 3350, G01N 3352, G01N 3354, G01N 2147

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active

044927525

ABSTRACT:
Method for discriminating unstained cells from stained cells in a heterogeneous population. Specified cell types are stained with an absorbing stain and all cells are passed through the class of flow cytometry instrumentation employing focused collimated light sources. Detection of low angle and wide angle light scatter permits differentiation between the cell types on the basis that cells stained with an absorbing stain produce comparatively less low angle light scatter and comparatively more wide angle light scatter than do unstained cells.

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