Method for discriminating between intact and damaged cells in a

Details Method for discriminating between intact and damaged cells in a Method for discriminating between intact and damaged cells in a

1988-06-13

1991-10-15

Kepplinger, Esther L.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 71, 435 72, 435 29, 435 34, 436 63, 436 172, 436800, C12Q 168, C12Q 102, C12Q 104, G01N 33535

Patent

active

050574132

ABSTRACT:
A method is disclosed for the discrimination between intact and damaged cells in a sample, and more particularly, the method uses a vital nucleic acid dye to selectively stain intact versus damaged cells which then may be counted and sorted by flow cytometry means. The method also may be used in conjunction with fluorescently labelled monoclonal antibodies to simultaneously identify cellular antigens.

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Herzenberg et al., Fluorescence Activated Cell Sorting, Sci. Am., 234:108 (1976).
Terstappan et al., Discriminating Between Damaged and Intact Cells in Fixed Flow Cytometric Samples, Cytometry, 9:477 (1988).
Practical Flow Cytometry, Shapiro, pp. 125-150 (Id. Ed., 1988) Alan R. Liss, Inc.
Rabinovitch et al., Journal of Immunology, 136:2769 (1986).

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