Method for discovering one or more peptides adapted for...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C435S006120, C435S005000, C435S004000, C435S091500, C435S091500, C435S091500

Reexamination Certificate

active

07033769

ABSTRACT:
A method for discovering one or more peptides adapted for specific binding to a microorganism of interest. The method comprises (i) identifying an antimicrobial peptide having antimicrobial activity against the microorganism of interest, (ii) generating a library of first generation mutants of the antimicrobial peptide, each of the first generation mutants differing from the antimicrobial peptide by a small number of amino acid substitutions, additions or deletions, (iii) screening the library of first generation mutants for those first generation mutants that bind to the microorganism of interest, (iv) determining the peptide sequences of those first generation mutants that bind to the microorganism of interest, and (v) if necessary, repeating steps (ii) through (iv) for one or more successive generations of mutants until one or more consensus peptide sequences emerge.

REFERENCES:
patent: 4683202 (1987-07-01), Mullis
patent: 5223408 (1993-06-01), Goeddel et al.
patent: 5447914 (1995-09-01), Travis et al.
patent: 5631228 (1997-05-01), Oppenheim et al.
patent: 5645996 (1997-07-01), Blondelle et al.
patent: 5646119 (1997-07-01), Oppenheim et al.
patent: 5659123 (1997-08-01), Van Rie et al.
patent: 5750357 (1998-05-01), Olstein et al.
patent: 5798336 (1998-08-01), Travis et al.
patent: 5885965 (1999-03-01), Oppenheim et al.
patent: 5889148 (1999-03-01), Lee et al.
patent: 5912230 (1999-06-01), Oppenheim et al.
patent: 5932701 (1999-08-01), Black et al.
patent: 6017728 (2000-01-01), Black et al.
patent: 6143498 (2000-11-01), Olsen et al.
patent: 6159719 (2000-12-01), Laine et al.
patent: 6191254 (2001-02-01), Falla et al.
patent: 6287804 (2001-09-01), Black
patent: 6287807 (2001-09-01), Wallis
patent: 6303771 (2001-10-01), Biswas et al.
patent: 6326462 (2001-12-01), Debouck et al.
patent: 6331411 (2001-12-01), Gwynn et al.
patent: 6335424 (2002-01-01), Black et al.
patent: 6335433 (2002-01-01), Biswas et al.
patent: 6346397 (2002-02-01), Warren, Jr. et al.
patent: 6350598 (2002-02-01), Wallis et al.
patent: 2001/0010912 (2001-08-01), Black et al.
patent: 2001/0016334 (2001-08-01), Wallis et al.
patent: 2001/0020010 (2001-09-01), Biswas et al.
patent: 2001/0041349 (2001-11-01), Patron et al.
patent: 2002/0004580 (2002-01-01), Fueyo et al.
patent: 2002/0004581 (2002-01-01), Palmer et al.
patent: 2002/0025537 (2002-02-01), Bylina et al.
patent: PCT/US99/00771 (1999-07-01), None
Epand et al., ‘Diversity of antimicrobial peptides and their mechanisms of action,’ Biochimica et Biophysica Acta, 1462:11-28 (1999).
Ostermeier et al., ‘A combinatorial approach to hybrid enzymes independent of DNA homology,’ Nature Biotechnology, 17:1205-9 (1999).
Sharma et al., ‘Semi-automated fluorogenic PCR assays (TagMan) for rapid detection ofEscherichia coliO157:H7 and other Shiga toxigenicE. coli,’ Molecular and Cellular Probes, 13:291-302 (1999).
Pyle et al., ‘Sensitive Detection ofEscherichia coliO157:H7 in Food and Water by Immunomagnetic Separation and Solid-Phase Laser Cytometry,’ Applied and Environmental Microbiology, 65(5) :1996-72 (1999).
Kuchner et al., ‘Directed evolution of enzyme catalysts,’ TIBTECH, 15 (12) :523-30 (1997).
Moore et al., ‘Directed evolution of a para-nitrobenzyl esterase for aqueous-organic solvents,’ Nature Biotechnology, 14:458-67 (1996).
Nicolas et al., ‘Peptides as Weapons Against Microorganisms in the Chemical Defense System of Vertebrates,’ Annu. Rev. Microbiol., 49:277-304 (1995).
Stemmer, ‘Rapid evolution of a protein in vitro by DNA shuffling,’ Nature, 370:389-91 (1994).
Lehtovaara et al., ‘A new method for random mutagenesis of complete genes: enzymatic generation of mutant libraries in vitro,’ Protein Engineering, 2(1) :63-8 (1998).

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Method for discovering one or more peptides adapted for... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for discovering one or more peptides adapted for..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for discovering one or more peptides adapted for... will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-3593029

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.