Method for direct rescue and amplification of integrated...

Chemistry: molecular biology and microbiology – Virus or bacteriophage – except for viral vector or... – Recovery or purification

Reexamination Certificate

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C435S320100, C435S325000, C435S366000, C435S369000, C435S183000, C435S235100, C435S005000, C435S006120, C435S237000

Reexamination Certificate

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10420284

ABSTRACT:
A method for isolating AAV viruses from cellular DNA of non-human primate (NHP) tissues by transfecting the DNA of NHP into 293 cells, rescuing the virus and amplifying it through serial passages in the presence of adenovirus helper functions is provided. Also provided are kits useful for performing this method.

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