Method for direct cloning of nuclease genes in E. coli

Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses

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43525233, 435199, C12N 1555, C12N 1570, C12N 922

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active

054985358

ABSTRACT:
The present invention discloses a novel method for the direct cloning of nuclease genes such as restriction endonuclease genes in E. coli. In addition, there is provided a novel strain which facilitates application of the method. This method has been successfully employed to clone a number of genes coding for endonuclease including restriction endonuclease genes.

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