Method for direct cloning and producing the BsoBI restriction en

Details Method for direct cloning and producing the BsoBI restriction en Method for direct cloning and producing the BsoBI restriction en

1994-12-15

1996-02-20

Patterson, Jr., Charles L.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435193, 4353201, 43525233, 536 232, C12N 922, C12N 1555

Patent

active

054928230

ABSTRACT:
The present invention discloses a novel method for the direct cloning of nuclease genes such as restriction endonuclease genes in E. coli. In addition, there is provided a novel strain which facilitates application of the method. This method has been successfully employed to clone a number of genes coding for endonuclease including restriction endonuclease genes.

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