Chemistry: analytical and immunological testing – Composition for standardization – calibration – simulation,... – Particle count or volume standard or control
Patent
1997-11-21
1999-02-23
Wallenhorst, Maureen M.
Chemistry: analytical and immunological testing
Composition for standardization, calibration, simulation,...
Particle count or volume standard or control
436 63, 436164, 436175, G01N 3100
Patent
active
058743103
ABSTRACT:
A method is provided for differentiation of nucleated red blood cells. In addition, the method provides for a concurrent differentiation of leukocytes in a blood cell sample by suitable electronic and optical measurements. The method includes exposing a blood cell sample to a reagent system to lyse mature red blood cells and subsequently analyzing nucleated red blood cells in a flow cell by optical analysis. A concurrent differentiation of nucleated blood cells and leukocytes can be performed using electronic and optical analysis. The electronic and optical analysis includes light scatter and impedance measurements. This method eliminates the use of nuclear stain for identification of nucleated red blood cells. The method of the present invention, for the first time, reports differentiation and enumeration of nucleated red blood cells without using fluorescence.
REFERENCES:
patent: 4284412 (1981-08-01), Hansen et al.
patent: 4735504 (1988-04-01), Tycko
patent: 5125737 (1992-06-01), Rodriguez et al.
patent: 5155044 (1992-10-01), Ledis et al.
patent: 5298426 (1994-03-01), Inami et al.
patent: 5389549 (1995-02-01), Hamaguchi et al.
patent: 5510267 (1996-04-01), Marshall et al.
patent: 5559037 (1996-09-01), Kim et al.
patent: 5631165 (1997-05-01), Chupp et al.
patent: 5648225 (1997-07-01), Kim et al.
patent: 5686308 (1997-11-01), Li et al.
patent: 5737078 (1998-04-01), Takarada et al.
patent: 5776709 (1998-07-01), Jackson et al.
Li Jing
Li Yi
Young Carole
Alter Mitchell E.
Coulter International Corp.
Wallenhorst Maureen M.
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