Method for diagnosing spinal muscular atrophy

Details Method for diagnosing spinal muscular atrophy Method for diagnosing spinal muscular atrophy

2011-01-25

2011-01-25

Babic, Christopher M. (Department: 1637)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

Reexamination Certificate

active

07875432

ABSTRACT:
A method for diagnosing spinal muscular atrophy is provided. The method includes providing a biological sample of a subject containing a nucleotide of SMN gene, amplifying SMN exons 1, 2a, 2b, 3, 4, 5, 6, 7, and 8 by a universal multiplex PCR using the nucleotide as a template and the primers to obtain fragments of the SMN exons 1, 2a, 2b, 3, 4, 5, 6, 7, and 8, labeling the fragments of the SMN exons 1, 2a, 2b, 3, 4, 5, 6, 7, and 8 by a fluorescent primer to obtain fluorescence-labeled exon fragments, and analyzing the fluorescence-labeled exon fragments by a capillary electrophoresis. If the SMN1/SMN2 ratios in exon 7 and 8 are different, it indicates that the subject is susceptible to spinal muscular atrophy. Additionally, if the peak of certain exon fragment appears crossed, it indicates an intragenic mutation in the exon.

REFERENCES:
Lorson et al. (Dev Dyn. Aug. 2008;237(8):2268-78).
Schmid et al. (J Child Neurol. Aug. 2007;22(8):1004-12).
Malcov et al. (Fetal Diagn Ther. Mar.-Apr. 2004;19(2):199-206).

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