Method for diagnosing for aneurysms

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism

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435 4, 435 721, 435 35, 4352402, 435244, 436 63, C12Q 102, C12Q 100, G01N 3353, C12N 500

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053407237

ABSTRACT:
The present invention pertains to a method for diagnosing for an aneurysm in a patient which comprises the steps of (a) explanting a skin section containing dermal fibroblast from the patient; (b) culturing the fibroblast to confluence in a culture medium; (c) incubating the cultured fibroblast with labeled proline to provide labeled procollagen in the culture medium; (d) separating the culture medium from the labeled procollagen and treating the labeled procollagen with a solution of protease inhibitor; (e) separating and purifying the labeled procollagen from the solution of protease inhibitor; (f) subjecting the labeled procollagen to protease digestion specific for non-collagenous proteins to form a collagenous mixture; (g) analyzing the collagenous mixture for the ratio of type I collagen to type III collagen; (h) analyzing a control collagenous mixture for the ratio of type I collagen to type III collagen; and (i) comparing the ratio of type I collagen to type III collagen in the collagenous mixture of step (g) to the control mixture of step (h), respectively.

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