Method for diagnosing cancer via DNA hybridization

Details Method for diagnosing cancer via DNA hybridization Method for diagnosing cancer via DNA hybridization

1998-01-16

2000-02-01

Hutzell, Paula K.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 4, 536 231, C12Q 168, C12Q 100, C07H 2102

Patent

active

060201347

ABSTRACT:
The invention described methods for identifying a molecule of interest, as well as nucleic acid molecules which encode it, and binding partners for it. A cDNA library from a cell expressing the target is prepared, and expressed in host cells. Lysates of the host cells are screened with a sample, treated to remove interfering binding partners. The treatment involves contact of the sample to lysates of untransfected host cells, and host cells transfected or transformed with the same vector used to make the cDNA library. Also a part of the invention are antigens and cDNA identified using the methodology.

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