Method for determining tyrosine kinase activity

Details Method for determining tyrosine kinase activity Method for determining tyrosine kinase activity

1996-11-01

1998-06-30

Houtteman, Scott W.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

530350, G01N 3353

Patent

active

057732370

ABSTRACT:
The subject invention provides for nucleotide sequences encoding polypeptide p62 and derivatives thereof. Another aspect of the subject invention also provides for methods of purifying p62 and derivatives thereof from cells naturally producing p62 and from cells genetically modified so as to produce p62.
The subject invention also provides for methods of assaying tyrosine kinase activity by means of measuring the phosphorylation of p62 and p62 derivatives. Measurement of p62/p62 derivative phosphorylation may be used to determine whether or not a call is cancerous.

REFERENCES:
Moran et al. (Cold Spring Harbor "Function and Evolution of RAS Proteins," Meeting Abstract, May 9-May 13, 1990.

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