Method for determining transferrin and composition therefor

Chemistry: analytical and immunological testing – Peptide – protein or amino acid – Glycoproteins

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436 84, 436910, G01N 3390

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active

045671508

ABSTRACT:
A method for determining the iron-binding capability of transferrin, consisting in adding to the sample being tested a reagent composed of a solution of a ferric salt adsorbed onto a suspension of magnesium carbonate, centrifuging the mixture so obtained and measuring the iron blocked by transferrin in the supernatant. The method is preferably carried out by utilizing a particular composition consisting of a single reagent composed of a suspension of a solution of a ferric salt absorbed on magnesium carbonate, possibly a medium capable of maintaining the pH between 8 and 9, if desired, a colorimetric reagent for the determination of iron.

REFERENCES:
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patent: 3887332 (1975-06-01), Takase et al.
patent: 4014651 (1977-03-01), Battinger et al.
patent: 4251360 (1981-02-01), Goldie et al.
Henry, "Clinical Chemistry--Principles and Technics", Harper & Row, 1964, pp. 391-395.
Ramsay, Clinica Chimica Acta, vol. 2, (1957), pp. 221-226.

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