Method for determining tissue of origin and degree of malignancy

Details Method for determining tissue of origin and degree of malignancy Method for determining tissue of origin and degree of malignancy

1985-12-24

1989-11-21

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

435 7, 435 29, 435264, 435268, 435269, 435270, 435272, 435 6, 436 63, 436 64, 436548, 530350, 530358, 530387, 530412, 530420, 935 78, 536 27, C12Q 168, C12Q 102, G01N 3353, C07G 1500

Patent

active

048822680

ABSTRACT:
A biochemical procedure for diagnosis of three important properties of cells in a biopsy or blood sample: tumor type i.e., the tissue type that has become neoplastic; tissue of origin if the tumor has arisen from a metastasis; and degree of malignancy. The procedure can also be used to obtain antibodies which can be used to determine tissue of origin by immunostaining and to detect tumor antigens appearing in blood by radioimmunoassay.
The procedure consists of isolating and analyzing components of a specific subcellular fraction referred to here as the "nuclear matrix". The nuclear matrix consists of proteins specific to different cell types and nuclear matrix associated DNA. The electrophoretic pattern of the proteins and restriction endonuclease digested DNA is unique and reproducible within a particular cell type and is therefore useful in diagnosing cell type. Changes in these patterns following transformation to a malignant phenotype provide additional diagnostic information.

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