Method for determining thioredoxin reductase activity

Details Method for determining thioredoxin reductase activity Method for determining thioredoxin reductase activity

1987-02-12

1989-07-18

Warden, Robert J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

435 4, 435173, 436173, 436803, 436 56, 436 63, 436 64, C12Q 126, C12Q 100, C12N 1300, G01N 2400

Patent

active

048493465

ABSTRACT:
The present invention provides a method for measuring the activity of thioredoxin reductase in mammalian cells by contacting the cells in vivo or in vitro with a quaternary ammonium salt spin-labelled with a stable nitroxide free radical, and measuring the rate of reduction of the uncomplexed nitroxide free radical at the cell surface by electron spin resonance. This rate of reduction provides a measure of thioredoxin reductase activity at the surface of the cells.

REFERENCES:
K. U. Schallreuter et al., Biochem. Biophys. Res. Commun., 136, 630 (1986).
K. U. Schallreuter et al., Biochem. Biophys. Res. Commun., 135, 221 (1986).

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