Method for determining the toxicity of selected organic chemical

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435962, 436518, 436523, 436524, 436526, 436531, 436534, 436824, G01N 3353, G01N 33543, G01N 33551, G01N 33546

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060177152

ABSTRACT:
A method for identifying a toxicant in an environmental water or soil sample comprising (a) adding to the sample an antibody known to form a complex with a toxicant suspected to be present in the sample; (b) incubating the antibody with the sample for a time and under conditions sufficient to allow complexes to form between the antibody and the toxicant; and (c) comparing the toxicity of the sample treated as in the step (b) with the toxicity of the sample prior to the step (a), whereby a reduced amount of toxicity of the treated sample compared with the untreated sample indicates the presence of the toxicant in said sample. The method described herein provides unexpected and important advantages by providing more accurate correlations between predicted and observed toxicity of a sample than previous methods.

REFERENCES:
patent: 5518890 (1996-05-01), Starkweather et al.
Lawruk et al., "Determination of Chlorothalonil in Water and Agricultural Products by a Magnetic Particle-Based Enzyme Immunoassay," Journal of Agricultural and Food Chemistry, vol. 43, pp. 1413-1419 (May 1995).
Kaufman et al., "Immunoassay of pesticides: an update," Journal of AOAC International, vol. 78, No. 4, pp. 1079-1090, (Jul.-Aug. 1995).
Herzog et al., "Immunochemical measurement of chlorpyrifos in multiple matrices," Journal of Clinical Ligand Assay, vol. 18, No. 3, pp. 150-155 (1995).

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