Chemistry: analytical and immunological testing – Heterocyclic carbon compound – Hetero-o
Patent
1991-03-06
1996-02-20
Redding, David A.
Chemistry: analytical and immunological testing
Heterocyclic carbon compound
Hetero-o
436171, 436173, 435 4, 422 70, 422 8205, 422 8209, G01N 3300, G01N 2100, F01N 310
Patent
active
054928362
ABSTRACT:
The invention provides a method for determining the structure of a carbohydrate sample, comprising perbenzoylating a carbohydrate sample with a perbenzoylating agent to protect free hydroxyl groups in the carbohydrate sample; cleaving the glycosidic linkages of the perbenzoylated carbohydrate sample by contacting the carbohydrate sample with an amount of BrCH.sub.2 COBr/H.sub.2 O effective to cleave the carbohydrate sample; treating the resulting product with AgOAc and methanol or AgOTf/TMU and methanol to effect glycosidation; treating the resulting product with thiourea to remove bromoacetate groups; subjecting the resulting product to effect methoxycinnamoylation of free hydroxyl groups; separating the resulting benzoates with high-pressure liquid chromatography; performing mass, ultraviolet and circular dichroic spectroscopy on the separated benzoates; and comparing the spectra so obtained with reference spectra or calculated values to identify the structure of the carbohydrate. The invention also provides an apparatus for automatically determining the structure of a carbohydrate molecule such as an oligosaccharide.
REFERENCES:
patent: 5104508 (1992-04-01), Williams et al.
Meyers Harold V.
Nakanishi Koji
Ojika Makoto
Wiesler William T.
Redding David A.
The Trustees of Columbia University in the City of New York
White John P.
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