Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1994-06-07
1998-06-02
Houtteman, Scott W.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 912, 536 2431, 536 2433, C12Q 168
Patent
active
057597723
ABSTRACT:
A method for determining the sex of a mammalian embryo is disclosed. This method begins with the isolation of an embryonic cell that contains pseudo-autosomal DNA sequences. At least a portion of the pseudo-autosomal DNA sequences are amplified, creating pseudo-autosomal DNA fragments. Allele-specific DNA sequences are amplified using the pseudo-autosomal DNA fragments as a template and allele-specific primers. The allele-specific products are examined for the presence or absence of the X-allele-specific and Y-allele-specific products. Preferably, the embryo is bovine and the pseudo-autosomal DNA sequences are ZFX and ZFY. Also preferably the allele-specific primers are those described in Sequence ID NOS: 5, 6, 7 and 8.
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Gyllensten in "PCR Technology," ed. by Henry A. Erlich, Stockton Press: New York. Chapter 5, pp. 45-60. (1989).
Arnheim in "PCR Technology," ed. by Henry A. Erlich, Stockton Press: New York. Chapter 12 pp. 119-135. (1989).
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Kirkpatrick Brian W.
Monson Ricky L.
Houtteman Scott W.
Wisconsin Alumni Research Foundation
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