Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving cholesterol
Patent
1992-11-24
1995-01-31
Fleisher, Mindy B.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving cholesterol
435 4, 435 15, 435 18, 435 19, 435 20, 435 21, 435 25, 435 26, 435 28, 435183, 435189, 435190, 435193, 435194, 435198, 435232, 435810, 435817, 435874, C12Q 160, C12Q 134, C12Q 144, C12Q 126
Patent
active
053858280
ABSTRACT:
The invention refers to a method for determining the relative amounts of all cholesterol-containing lipoproteins in body fluids comprising electrophoretically separating the lipoproteins of an aliquot of body fluid on a thin layer carrier matrix, incubating the carrier matrix, containing the separated lipoproteins with cholesterol esterase and cholesterol dehydrogenase, forming a provable complex, and determining the relative amounts of the different lipoprotein classes2. The new method makes it possible to simultaneously determine HDL-, LDL-, VLDL- and LP (X)-cholesterol in body fluids with a high accuracy even at small concentrations. The thin layer matrices obtained electrophoretically, are very easy to handle and to record.
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Aufenanger et al., "A specific method for the direct determination of lipoprotein cholesterol in electrophoretic patterns", Biochem. Methods 109:421, Abstract No. 226121w (1988).
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Fleisher Mindy B.
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