Method for determining the presence or concentration of a bound

Details Method for determining the presence or concentration of a bound Method for determining the presence or concentration of a bound

1988-03-29

1994-10-11

Kepplinger, Esther L.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 79, 435 792, 435803, 435962, 436541, 436824, 422 59, 422 70, C12Q 168, G01N 3353, G01N 33538

Patent

active

053546555

ABSTRACT:
There is disclosed a process and a device for detecting and measuring (1) the amount of enzyme present as a detecting system following a nucleic acid hybridization reaction or immunoreaction; (2) the level and activity of free enzyme in a biological sample; (3) the level of enzyme from contaminating microorganisms present in a sample; and (4) enzymes from pure culture isolates for microbial identification and antimicrobial susceptibility testing.

REFERENCES:
patent: 4039652 (1977-08-01), Adams et al.
Kuichi et al., Chemical Abstract 101(11): 86119; "A fluorometric microassay for monitoring the enzymic activity of GMI-ganglioside.beta.-galactosidase by use of high performance liquid chromatography", Anal. Biochem., 140(1), 146-51, 1984.

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