Method for determining the presence or amount of analyte using a

Details Method for determining the presence or amount of analyte using a Method for determining the presence or amount of analyte using a

1994-05-12

1996-06-25

Scheiner, Toni R.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 71, 435 72, 435962, 435970, 436518, 436524, 436810, 252302, C12Q 168

Patent

active

055299016

ABSTRACT:
This invention relates to a method for determining the presence or amount of an analyte in a sample comprising contacting the sample with a labeled constituent consisting of an aqueous carbon sol which is stable in the absence of a stabilizing substance. The colloidal carbon particles have directly conjugated to their surface a binding component which specifically binds to the analyte and the resulting analyte/carbon particle complex is used as an indication of the presence or amount of analyte in the sample. The grade of carbon used satisfies the condition V>0 wherein V is a linear predictor value according to the formula V=-138.954-0.987.times.DBP+15,609.times.VC+3.994.times.PPD, wherein DBP is the dibutylphthalate adsorption in ml/100 g, as determined according to DIN 53601; VC is the volatile content in %, as determined according to DIN 53552; and PPD is the average primary particle diameter in nanometers.

REFERENCES:
T. Waller, "The india-ink immunoreaction: a method for the rapid diagnosis of encephalitozoonosis"; Laboratory Animals (1977) II, 93-97.
P. Geck, "India-Ink Immuno-Reaction for the Rapid Detection of Enteric Pathogens"; Acta microbiol. Acad. Sci. hung. 18: 191-196 (1971).
N. R. Berguist et al, "A Novel Simple Immunoassay for Rapid Detection of Human IgG antibodies to Toxoplasma gondii"; J. Immunolog. Method, 61: 339-344 (1983).

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