Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2006-07-04
2006-07-04
Lacourciere, Karen A. (Department: 1635)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C536S024300
Reexamination Certificate
active
07070925
ABSTRACT:
The present invention concerns oligonucleotides containing one or more modified nucleotides which increase the binding affinity of the oligonucleotides to target nucleic acids having a complementary nucleotide base sequence. These modified oligonucleotides hybridize to the target sequence at a faster rate than unmodified oligonucleotides having an identical nucleotide base sequence. Such modified oligonucleotides include oligonucleotides containing at least one 2′-O-methylribofuranosyl moiety joined to a nitrogenous base. Oligonucleotides can be modified in accordance with the present invention to preferentially bind RNA targets. The present invention also concerns methods of using these modified oligonucleotides and kits containing the same.
REFERENCES:
patent: 4725536 (1988-02-01), Fritsch et al.
patent: 4766062 (1988-08-01), Diamond et al.
patent: 4797355 (1989-01-01), Stabinsky
patent: 4820630 (1989-04-01), Taub
patent: 4822733 (1989-04-01), Morrison
patent: 4925785 (1990-05-01), Wang et al.
patent: 4996143 (1991-02-01), Heller et al.
patent: 5087558 (1992-02-01), Webster, Jr.
patent: 5091519 (1992-02-01), Cruickshank
patent: 5118801 (1992-06-01), Lizardi et al.
patent: 5210015 (1993-05-01), Gelfand et al.
patent: 5403711 (1995-04-01), Walder et al.
patent: 5445933 (1995-08-01), Eadie et al.
patent: 5487972 (1996-01-01), Gelfand et al.
patent: 5514546 (1996-05-01), Kool
patent: 5514551 (1996-05-01), Yang et al.
patent: 5536638 (1996-07-01), Rossau et al.
patent: 5573906 (1996-11-01), Bannwarth et al.
patent: 5574145 (1996-11-01), Barry et al.
patent: 5607834 (1997-03-01), Bagwell
patent: 5641625 (1997-06-01), Ecker et al.
patent: 5645985 (1997-07-01), Froehler et al.
patent: 5652099 (1997-07-01), Conrad
patent: 5656427 (1997-08-01), Hammond et al.
patent: 5679553 (1997-10-01), Van Gemen et al.
patent: 5691146 (1997-11-01), Mayrand
patent: 5723591 (1998-03-01), Livak et al.
patent: 5731148 (1998-03-01), Becker et al.
patent: 5747252 (1998-05-01), Yang et al.
patent: 5808023 (1998-09-01), Sanghvi et al.
patent: 5811533 (1998-09-01), Gold et al.
patent: 5827649 (1998-10-01), Rose et al.
patent: 5846726 (1998-12-01), Nadeau et al.
patent: 5849504 (1998-12-01), Shah et al.
patent: 5866336 (1999-02-01), Nazarenko et al.
patent: 5898031 (1999-04-01), Crooke et al.
patent: 5914396 (1999-06-01), Cook et al.
patent: 5919630 (1999-07-01), Nadeau et al.
patent: 5925517 (1999-07-01), Tyagi et al.
patent: 5928862 (1999-07-01), Morrison
patent: 6001564 (1999-12-01), Bergeron et al.
patent: 6020130 (2000-02-01), Gold et al.
patent: 6030787 (2000-02-01), Livak et al.
patent: 6117635 (2000-09-01), Nazarenko et al.
patent: 6130038 (2000-10-01), Becker et al.
patent: 6150097 (2000-11-01), Tyagi et al.
patent: 6156501 (2000-12-01), McGall et al.
patent: 6218107 (2001-04-01), Brentano et al.
patent: 6410276 (2002-06-01), Burg et al.
patent: 0318245 (1989-05-01), None
patent: 0415901 (1991-03-01), None
patent: 0421725 (1991-04-01), None
patent: 0742287 (1996-11-01), None
patent: 9012116 (1990-10-01), None
patent: 9014442 (1990-11-01), None
patent: 9108480 (1991-06-01), None
patent: 9202258 (1992-02-01), None
patent: 9313121 (1993-07-01), None
patent: WO94/01550 (1994-01-01), None
patent: 9402501 (1994-02-01), None
patent: 9415169 (1994-07-01), None
patent: 9419023 (1994-09-01), None
patent: 9514706 (1995-06-01), None
patent: 9522623 (1995-08-01), None
patent: 9532305 (1995-11-01), None
patent: 9606950 (1996-03-01), None
Shimizu et al., Bioorganic & Medicinal Chemistry Letters, 1994, vol. 4, No. 8, pp. 1029-1032.
Inoue et al., Nucleic Acids Research 1987, vol. 15, No. 15, pp. 6131-6148.
Iribarren et al., 2′-O-Alkyl Oligoribonucleotides as Antisense Probes, Proc. Natl. Acad. Sci. USA, Oct. 1990, vol. 87, pp. 7747-7751.
Barabino et al., “Targeted snRNP Depletion Reveals an Additional Role for Mammalian U1 snRNP in Spliceosome Assembly,” Cell, 63:293-302 (1990).
Blencowe et al., “Antisense Probing of the Human U4/U6 snRNP with Biotinylated 2′-OMe RNA Oligonucleotides,” Cell, 59:531-539 (1989).
Carmo-Fonseca et al., “In vivodetection of snRNP-rich organelles in the nuclei of mammalian cells,” EMBO, 10(7):1863-1873 (1991).
Carmo-Fonseca et al., “Assembly of snRNP-containing Coiled Bodies Is Regulated in Interphase and Mitosis-Evidence that the Coiled Body Is a Kinetic Nuclear Structure,”J.Cell.Biol.,120(4):841-852 (1993).
Carmo-Fonseca et al., “Transcription-dependent Colocalization of the U1, U2, U4/U6 and U5 snRNPs in Coiled Bodies,” J.Cell.Biol., 117(1):1-14 (1992).
Carmo-Fonseca et al., “Mammalian nuclei contain foci which are highly enriched in compnnents of the pre-mRNA splicing machinery.” EMBO, 10(1):195-206 (1991).
Lamond et al., “Probing the Structure and Function of U2 snRNP with Antisense Oligonucleotides Made of 2′-OMe RNA,” Cell,58:383-390 (1989).
Palfi et al., “Affinity purification of Trypanosoma brucei small nuclear ribonucleoproteins reveals common and specific protein components,” Proc.Natl.Acad.Sci.USA, 88:9097-9101 (1991).
Wolff et al., “Reconsituted mammalian U4/U6 snRNP complements splicing: a mutational analysis,” EMBO, 11(1):345-359 (1992).
Azhayeva et al., “Selective Binding of Looped Oligonucleotides to a Single-Stranded DNA and its Influence of Replication in vitro”, Nucleic Acids Research, Nov. 1995, 23(21):4255-4261.
Lubini et al., “Stabilizing Effects of the RNA 2′-Substituent: Crystal Structure of an Oligodeoxynucleotide Duplex Containing 2′-O'Methylated Adenosines”, Chemistry & Biology, Sep. 1994, 1(1):39-45.
Refregiers et al., “Fluorescence Resonance Energy Transfer Analysis of the Degradation of an Oligonucleotide Protected by a Very Stable Hairpin”, Journal of Biomolecular Structure & Dynamics, Dec. 1996, 14(3):365-371.
Bobst et al., “Effect of the Methylation of the 2′-Hydroxyl Groups in Polyadenylic Acid on its Structure in Weakly Acidic and Neutral Solutions and on its Capability to Form Ordered Complexes with Polyuridylic Acid”,J. Mol. Biol., 46:221-234 (1969).
Burd et al., “Conserved Structures and Diversity of Functions of RNA-Binding Proteins”,Science, 265:615-621 (1994).
Chiang et al., “Antisense Oligonucleotides Inhibit Intercellular Adhesion Molecular 1 Expression by Two Distinct Mechanisms”,J. Biol. Chem., 266(27):18162-18171 (1991).
Corey, “48000-fold Acceleration of Hybridization by Chemically Modified Oligonucleotides”,J. Am. Chem. Soc., 117(36):9373-9374 (1995).
Cummins et al., “Characterization of Fully 2′-modified Oligoribonucleotide Hetero- and Homoduplex Hybridization and Nuclease Sensitivity”,Nucleic Acids Research, 23(11):2019-2024 (1995).
Dean et al., “Inhibition of Protein Kinase C-α Expression in Human A549 Cells by Antisense Oligonucleotides Inhibits Induction of Intercellular Adhesion Molecule 1 (ICAM-1) mRNA by Phorbol Esters”,J. Biol. Chem., 269(23):16416-16426 (1991).
Ecker et al., “Pseudo-Half-Knot Formation with RNA”,Science, 257:958-961 (1992).
Goodchild, “Conjugates of Oligonucleotides and Modified Oligonucleotides: A Review of Their Synthesis and Properties”,Bioconjugate Chemistry, 1(3):165-187 (1990).
Hou et al., “Inhibition of tRNA Aminoacylation by 2′-O-Methyl Oligonucleotides”,Biochemistry, 35(48):15340-15348 (1996).
Inoue et al., “Sequence-dependent.hydrolysis of RNA using modified oligonucleotide splints and RNase H”,FEBS Letter, 215(2):327-330, 1987.
Inoue et al., “Synthesis and hybridization studies on two complementary nona(2′-O-methyl)ribonucleotides”,Nucleic Acids Research, 15(15):6131-6148 (1987).
Iribarren et al., “2′-O-Alkyl oligoribonucleotides as antisense probes”,Proc. Natl. Acad. Sci, USA, 87:7747-7751 (1990).
Knorre et al., “Oligonucleotides Linked to Reactive Groups”,Oligo
Becker Michael M.
Brentano Steven T.
Majlessi Mehrdad
Cappellari Charles B.
Fisher Carlos A.
Gen-Probe Incorporated
Lacourciere Karen A.
LandOfFree
Method for determining the presence of an RNA analyte in a... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for determining the presence of an RNA analyte in a..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for determining the presence of an RNA analyte in a... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3598296