Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2008-05-27
2008-05-27
Chunduru, Suryaprabha (Department: 1637)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S091200, C536S024330
Reexamination Certificate
active
07378241
ABSTRACT:
The present invention concerns a method for determining the efficiency of the amplification of a target nucleic acid comprising the following steps: (i) preparation of a dilution series of the target nucleic acid, (ii) amplifying the target nucleic acid under defined reaction conditions, the amplification being measured in real-time (iii) setting a defined signal threshold value, (iv) determining the cycle number at which the signal threshold value is exceeded for various dilutions, (v) determining the amplification efficiency as a function of the amount of original target nucleic acid. The present invention also concerns a method for the quantification of a target nucleic acid in a sample in which the efficiency of the amplification reaction is determined in this manner and is taken into account in the quantification.
REFERENCES:
patent: 2516655 (1950-07-01), Smith
patent: 3163404 (1964-12-01), Kraft et al.
patent: 3614434 (1971-10-01), Horwitz
patent: 3711379 (1973-01-01), Adams
patent: 5499872 (1996-03-01), Baxter
patent: 5580524 (1996-12-01), Forrest et al.
patent: 5858658 (1999-01-01), Haemmerie et al.
patent: 6143496 (2000-11-01), Brown et al.
patent: 6174670 (2001-01-01), Wittwer et al.
patent: 6232079 (2001-05-01), Wittwer et al.
patent: 6235504 (2001-05-01), Zhang et al.
patent: 6691041 (2004-02-01), Sagner et al.
patent: 7125691 (2006-10-01), Sagner et al.
patent: 2002/0058262 (2002-05-01), Sagner et al.
patent: 0 640 828 (1994-08-01), None
patent: 0 959 140 (1999-06-01), None
patent: 1138784 (2001-02-01), None
patent: 01 10 7737 (2003-02-01), None
patent: 01 10 7738 (2003-03-01), None
patent: WO 97/46707 (1997-12-01), None
patent: WO 99/54510 (1999-10-01), None
patent: WO 9954510 (1999-10-01), None
Bourinbaiar et al., “Simple Procedure For Estimating The Efficiency of PCR,” Molecular Biotechnology (1996) 6:87-90.
Fujii et al., “Mutation Detection by TaqMan-Allele Specific Amplification: Application to Molecular Diagnosis of Glycogen Storage Disease Type Ia and Medium Chain Acyl-CoA Dehydrogenase Deficiency,” Human Mutation (2000) 15:189-196.
Isono K. “A Novel Quantitative PCR With Fluorogenic Probe,” The Japanese Journal of Clinical Pathology, (1997) 45 218-223 Abstract.
LightCycler System. Roche Molecular Biochemicals (1999) 1-6.
Rodriguez-Antona et al., “Quantitative RT-PCR Measurement of Human Cytochrome P-450s: Application to Drug Induction Studies,” (2000) Archives of Biochemistry and Biophysics 376:109-116.
Gibson, Ursula E. M. et al.; “A Novel Method for Real Time Quantitative RT-PCR”; 1995,Genome Research, vol. 6, pp. 995-1001.
Meijerink et al. A novel method to compensate for different amplification efficiencies between patient DNA samples in quantitative real-time PCR.J Mol Diagnostics, vol. 3, No. 2, pp. 55-61, 2001.
Gutekunst Martin
Sagner Gregor
Soong Richie
Tabiti Karim
Chunduru Suryaprabha
Doyle Charles M.
Roche Diagnostics Operations Inc.
Townsend and Townsend / and Crew LLP
LandOfFree
Method for determining the efficiency of nucleic acid... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for determining the efficiency of nucleic acid..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for determining the efficiency of nucleic acid... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2767643