Radiant energy – Ionic separation or analysis – Methods
Reexamination Certificate
2008-07-14
2011-10-25
Nguyen, Kiet (Department: 2881)
Radiant energy
Ionic separation or analysis
Methods
C435S007500, C514S04400A, C530S300000
Reexamination Certificate
active
08044345
ABSTRACT:
The present invention relates to a method for determining the concentration of a first molecule having a chemical structure, which contains a first peptide sequence comprising:(a) obtaining a sample containing the first molecule, (b) providing a reference sample which contains a second molecule having a certain concentration and chemical structure, which contains a second peptide sequence, wherein the chemical structure of the second molecule only differs from the structure of the first molecule in one or more permutations in the first peptide sequence, (c) combining the reference sample and the sample containing the first molecule, (d) identifying at least one fragment peak in a mass spectrum (MS) of the first molecule and the second molecule, wherein the mass difference of the fragment peaks is only caused by the permutation of the at least two different amino acids, and (e) determining the concentration of the first molecule relative to the certain concentration of the second molecule by comparing of the identified peaks.
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Gerber, S. et al. “Absolute quantification of proteins and phosphoproteins from cell lysates by tandem MS”, PNAS, Jun. 10, 2003, pp. 6940-6945, vol. 100, No. 12.
Oda, Y. et al. “Accurate quantitation of protein expression and site-specific phosphorylation”, PNAS, vol. 96, pp. 6591-6596, Jun. 1999.
Bucknall, M. et al “Practical Quantitative Biomedical Applications of MALDI-TOF Mass Spectrometry”, J. Am. Soc. Mass Spectrom 2002, vol. 13, pp. 1015-1027.
Van Der Rest, G. et al. “Gas-Phase Cleavage of PTC-Derivatized Electrosprayed Tryptic Peptides in an FT-ICR Trapped-Ion Cell: Mass-Based Protein Identification without Liquid Chromatographic Separation”, J. Am. Soc. Mass Spectrom 2001, vol. 12, pp. 288-295.
Written Opinion dated Mar. 9, 2008.
Lehmann Wolf Dieter
Winter Dominic
Birch & Stewart Kolasch & Birch, LLP
Deutsches Krebsforschungszentrum
Nguyen Kiet
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