Method for determining the coagulation potential of a plasma...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving blood clotting factor

Reexamination Certificate

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C436S069000

Reexamination Certificate

active

06838251

ABSTRACT:
A method of determining the coagulation potential of a plasma sample be pre-incubating the plasma sample with a reagent such that endogenous protein C in the plasma is at least partially converted into activated protein C by the reagent, adding factor Xa which is progressively inactivated by antithrombin III/heparin cofactor 2 during the preincubation, adding an exogenous reagent which activates factor X to Xa or prothrombin to thrombin in a factor V-dependent manner, monitoring a reaction indicative of the rate of coagulation of the plasma sample, comparing that rate of coagulation control, or the equivalent rate determined for an individual without impaired coagulation control, or the equivalent rate determined for the plasma sample in the absence of protein C activator, and determining the coagulation potential of the plasma sample from one or other of the compairsons.

REFERENCES:
patent: 5601995 (1997-02-01), Exner
patent: 5643739 (1997-07-01), Varadi et al.
patent: 5726028 (1998-03-01), Kraus
patent: 5780255 (1998-07-01), Preda
patent: 6051434 (2000-04-01), Exner
patent: 6251619 (2001-06-01), Van Dreden
patent: 6978587 (1987-09-01), None
patent: 3724443 (1989-02-01), None
patent: WO 9101382 (1991-02-01), None
Thromb, Haemost, Mar. 1997, 77(3) pp. 436-439, Armando Tripodie et al, Screening for the FV:Q506 Mutation- Evaluation of Thirteen Plasma Based Methods for their Diagnostic Efficacy in Comparison with DNA Analysis; pp. 436-437 (methods section) and p. 438 col. 2 lines 1-5.
Thrombosis Research 1997 87(6) pp. 501-510 Katharina Ruzicka et al., Evaluation of a New Screening Assay Proc® Global Identification of Defects in the Protein C/protein S Anticoagulant Pathway.

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