Method for determining the biologically effective...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Reexamination Certificate

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C435S007200

Reexamination Certificate

active

07851163

ABSTRACT:
Immunoassay for the determination of parathyroid hormone activity in a sample, and for the diagnosis, etiology and treatment of calcium metabolism disturbances, osteopathies and hyper- or hypoparathyroidisms. The parathyroid hormone activity is measured with the aid of an antibody which binds to an epitope in the region of the receptor binding structure15to22of the parathyroid hormone, and an antibody which recognizes whether the amino-terminal end1to3of the parathyroid hormone is intact. The assay permits the antagonistic characteristics of some parathyroid hormone fragments to be taken into account.

REFERENCES:
patent: 6030790 (2000-02-01), Adermann et al.
patent: 6689566 (2004-02-01), Cantor et al.
patent: WO 96/10041 (1996-04-01), None
Gardella et al. Endocrinology 1993 vol. 132, p. 2024-30.
Pellegrini et al. Biochemistry 1998 vol. 37, p. 12737-43.
Barbier et al. Biochemistry 2000 vol. 39, p. 14522-30.
Blind, E. et al. Measurement of intact human parathyrin by an extracting two-site immunoradiometric assay.Clinical Chemistry, 1987;33(8):1376-1381.
Mägerlein, M. et al. A new immunoenzymometric assay for bioactive N-terminal human parathyroid hormone fragments and its application in pharmacokinetic studies in dogs.Arzneimittelforschung, Feb. 1998;48(2):199-204.
Re-examination of US-6,030,790, Control No. 90007412.

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