Method for determining steroids in human body liquids

Details Method for determining steroids in human body liquids Method for determining steroids in human body liquids

1978-03-16

1980-10-28

Hunt, Brooks H.

Drug, bio-affecting and body treating compositions

Radionuclide or intended radionuclide containing; adjuvant...

Molecular bilayer structure

424 12, 23230B, 232306, 422 61, A61K 4300, G01N 3316, B65D 8132

Patent

active

042306841

ABSTRACT:
A micromethod for the determination of steroids in human body liquids, in particular blood, is disclosed. A small sample of the liquid is transferred to a sheet of absorbing material, e.g., filter paper. A disc of the impregnated paper, containing the dried sample, in eluted with an aqueous solvent, e.g., a buffer solution. Without separating the paper from the elute, the latter is extracted with an organic solvent. A steroid-containing residue is recovered from the organic extract and is subjected to a conventional radioimmunoassay. The method is particularly suited for the detection of disorders with elevated steroid levels such as congenital adrenal hyperplasia in newborn infants by determining the 17.alpha.-hydroxy-progesterone concentration in small blood samples.

REFERENCES:
patent: 4018885 (1977-04-01), Bohn et al.
patent: 4081525 (1978-03-01), Knight et al.
James, In Radioimmuno Assay of Steroid Hormones, Ed. Gopta., Verlag Chemie, Weinheim, 1975, pp. 1-8.
Bruno, In New Techniques in Tumor Location and Radioimmuno Assay, Ed. Crollgtal, J. Wiley & Sons, Inc., N.Y., 1974, pp. 9-15.

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