Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage
Patent
1993-12-02
2000-01-11
Arthur, Lisa B.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving virus or bacteriophage
435 6, C12Q 170, C12Q 168
Patent
active
060134313
ABSTRACT:
Detection of variable nucleotide(s) is based on primer extension and incorporation of detectable nucleoside triphosphates. By selecting the detection step primers from the region immediately adjacent to the variable nucleotide, this variation can be detected after incorporation of as few as one nucleoside triphosphate. Labelled nucleoside triphosphates matching the variable nucleotide are added and the incorporation of a label into the detection step primer is measured. The selection of the detection step primer is important to the method according to this invention and is dependent on the nucleotide sequence of interest. The detection step primers are preferably selected so as to span the region immediately toward the 3' end from the variable nucleotide to be detected. The detection step primers can also be complementary to a sequence beginning several nucleotides removed from the variable nucleotide. The only limitation concerning the position of the detection step primers is that the sequence between the 3' end of the detection step primer and the variable nucleotide to be detected must not contain a nucleotide residue of the same type as the one to be detected. The detection step primers can equally well be chosen to be complementary to either the coding or non-coding strands of the nucleotide sequence of interest.
REFERENCES:
patent: 4656127 (1987-04-01), Mundy
patent: 4683202 (1987-07-01), Mullis
patent: 4800159 (1989-01-01), Mullis et al.
patent: 4840892 (1989-06-01), Adams
patent: 4851331 (1989-07-01), Vary et al.
patent: 4863849 (1989-09-01), Melamede
Syvanen et al. (1990) Genomics 8; 684-692.
Mahley, "Apolipoprotein E: Cholesterol Transport Protein With Expanding Role in Cell Biology", Science, 240:622-630 (1988).
Thomson, "HLA Disease Associations: Models for Insulin Dependent Diabetes Mellitus on the Study of Complex Human Genetic Disorders", Annu. Rev. Genet., 22:31-50 (1988).
Morel et al., "Aspartic Acid at Position 57 of the HLA-DQ .beta. Chain Protects Against Type I Diabetes: A Family Study", Proc. Natl. Acad. Sci. USA, 85:8111-8115 (1988).
Scharf et al., "HLA Class II Allelic Variation and Susceptibility to Pemphigus Vulgaris", Proc. Natl. Acad. Sci. USA, 85:3504-3508 (1988).
Farr et al., "Analysis of RAS Gene Mutations in Acute Myeloid Leukemia by Polymers Chain Reaction and Oligonucleotide Probes", Proc. Natl. Acad. Sci. USA, 85:1629-1633 (1988).
Kerem et al., "Identification of the Cystic Fibrosis Gene: Genetic Analysis", Science, 245:1073-1080 (1989).
Antonarakis, "Diagnosis of Genetic Disorders at the DNA Level", N. Engl. J. Med., 320:153-163 (1989).
Bos et al., "Prevalence of ras Gene Mutations in Human Colorectal Cancers", Nature 327:293-297 (1987).
Kleppe et al., "Studies on Polynucleotides", J. Mol. Biol., 56:341-361 (1971).
Mullis & Faloona (1987) Specifi. Synthesis of DNA in vitro v.s. Polymerase-Catalyzed Chem. Reaction. Meth. in Enzymol. v. 155, p. 335.
McMahon et al. (1987) Characterization of c-Ki-ras oncogene . . . Proc. Natl. Acad. Sci. USA (1987) vol. 84 pp. 4974-4978.
McLean et al. (1984) human Apoliprotein E mRNA . . . J. Biol. Chem. Vo. 259 (10) pp. 6498-6504.
Mullis (Apr. 1990) Scientific American, pp. 56-65.
Ralls et al. (1989) Journal of Clinical Investigation, vol. 83, pp. 1095-1101.
Soderlund Hans E.
Syvanen Anne-Christine
Arthur Lisa B.
Auerbach Jeffrey I.
Molecular Tool, Inc.
LandOfFree
Method for determining specific nucleotide variations by primer does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for determining specific nucleotide variations by primer , we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for determining specific nucleotide variations by primer will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1461070