Method for determining nucleotide identity through extension of

Details Method for determining nucleotide identity through extension of Method for determining nucleotide identity through extension of

1991-10-11

1999-12-21

Arthur, Lisa B.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

435 6, 435 912, 435 231, C12Q 170, C12Q 168, C12P 1934, C07H 2104

Patent

active

060047445

ABSTRACT:
The invention concerns a reagent composition that employs at least two different terminators of a nucleic acid template-dependent primer extension reaction to determine the identity of a nucleotide base at a specific position in a nucleic acid of interest. The invention also concerns an immobilized method for determining such identification. The invention may be used to determine the presence or absence of a specific nucleotide sequence in a sample. It may also be employed in determination of genotype and in the identification of different alleles.

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