Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Reexamination Certificate
2006-04-04
2006-04-04
Ceperley, Mary E. (Department: 1641)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
C435S007100, C435S007200, C435S006120, C436S544000, C436S546000, C436S172000, C436S824000, C436S905000, C436S161000, C436S173000, C436S094000, C530S405000, C530S412000
Reexamination Certificate
active
07022491
ABSTRACT:
The invention relates to a method for monitoring interactions to a target biomolecule comprising the steps of: providing a biomolecule of interest having specificity for the target biomolecule; binding the biomolecule of interest to at least one type of linker molecule comprising a unique mass marker part; introducing the biomolecule of interest to a cell; binding the linker to the target biomolecule; cleaving the linker molecule, thereby leaving the photoactivatable part and the mass marker part bound to the target; analysing the target biomolecule, thereby detecting the unique mass marker part. The detection can be carried out by MS in a parent ion scanning mode, thereby allowing study of the interaction between the biomolecule of interest and the target biomolecule.
REFERENCES:
patent: 4487838 (1984-12-01), Hynes et al.
patent: 0 778 280 (2003-01-01), None
patent: WO 00/02893 (2000-01-01), None
Blackstock, W. P., et al., “Proteomics: quantitative and physical mapping of cellular proteins” Trends in Biotechnology, Elsevier, Amsterdam, NL vol. 17, No. 3, Mar. 1999 pp. 121-127.
Oatis, Jr., J. E., et al., “Synthesis and Photochemistry of Two Cleavable Heterobifunctional Benzophenone Protein Crosslinkers” Tetrahedron Letters, Elsevier Sciences, Ltd. vol. 39, No. 13, 1998 pp. 1665-1668.
Ceperley Mary E.
Haq Shafiqul
Ji Yonggang
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