Chemistry: natural resins or derivatives; peptides or proteins; – Peptides of 3 to 100 amino acid residues
Patent
1992-10-29
1999-12-07
Sisson, Bradley
Chemistry: natural resins or derivatives; peptides or proteins;
Peptides of 3 to 100 amino acid residues
530329, 530330, 530333, 530334, 530810, C07K 106, C07K 400, C07K 510, C08G 6910, C08G 6902
Patent
active
059985771
ABSTRACT:
A method of determining the sequence of monomers which is a conformational equivalent of an epitope which is complementary to a particular paratope of an antibody of interest, the method comprising the steps of: 1. synthesizing a plurality of catamer preparations; each of said catamer preparations consisting of a plurality of catamers in which the composition at one or more designated positions in each catamer is known, and the composition at the remaining positions is randomly made up from members of a defined set of monomers; and said plurality of catamer preparations comprising preparations in which the composition at the designated positions is systematically varied to contain members from a defined set of monomers; 2. contacting each of the plurality of catamer preparations with the antibody of interest; and 3. detecting the presence or absence of binding between each of said plurality of catamer preparations and said given antibody to indicate a partial sequence of the mimotopes for the paratope of the said given antibody.
REFERENCES:
patent: 5420246 (1995-05-01), Rutter et al.
Geysen et al (1984) Proc. Nat. Acad Sci, USA 81 : 3998-4002.
Smith, J.A., Hurrell, J.G.R. and Leach, S.J., "A Novel Method for Delineating Antigenic determinants: Peptide Synthesis and Radioimmunassy Using the Same Solid Support", Immunochemistry, 24; 565-568, 1977.
Houghten, R.A., "General Method for the Rapid Solid-phase Synthesis of large Numbers of Peptides: Specificity of Antigen-antibody Interaction at the Level of Individual Amino Acids", Proc. Nat'l. Acad. Sci, USA, 82: 5131-5135, 1985.
Kent, S., Neurath, A.R. Aebersold, R., Parker, K. & Strick, N., "Epitope scanning--Strategies for Determining the Precise Location of Continuous Epitopes Applied to the pre-S Region of HBV", Cold Spring Harbor Symposium, Modern Approaches to Vaccines,Sep. 1985.
Bugaisky Gabriele E.
Chiron Mimotopes Pty. Ltd.
Sisson Bradley
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