Method for determining manganese level in blood using a porphyri

Details Method for determining manganese level in blood using a porphyri Method for determining manganese level in blood using a porphyri

1990-01-23

1992-04-21

Lacey, David L.

Chemistry: analytical and immunological testing

Metal or metal containing

Present in biological fluids

424617, 514 22, 514183, 514184, G01N 3370

Patent

active

051067536

ABSTRACT:
The present invention is directed to a simple and inexpensive liquid or dry reagent assay system for detecting nanomolar amounts of manganese in body fluids in microgram per liter concentrations. More specifically, the manganese assay of this invention is directed to the optical detection of a chromogenic reaction, such as the oxidation of a redox indicator, catalyzed by a manganese porphyrin complex.

REFERENCES:
Masaaki Tabata; "Kinetic Method for the Determination of Nanogram Amounts of Lead (II) Using its Catalytic Effect on the Reaction of Manganese (II) with 5, 10, 15, 20-tetrakis (4-sulphanotophenyl)porphine"; Analyst, Feb. 1987 vol. 112 pp. 141-144.
Gelb, M. H.; Toscano, W. A.; Shgar, S. G.; "Chemical mechanisms for cytochrome P-450 oxidation: Spectral an catalytic properties of a manganese-substituted protein"; Proc. Natl. Acad. Sci. USA, vol. 79, pp. 5758-5762, Oct. 1982
Hagime Ishii; Hidemasa Koh; Katcuhiko Satoff; "Spectrophotometric determination of Manganese Utilizing Metal Ion Substitution in the Cadmium-.alpha.,.beta.,.gamma.,.delta.-Tetrakis(4-Carboxyphenyl)Porphine Complex", Analytica Chimica Acta, 136(1982) 347-352.

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