Chemistry: analytical and immunological testing – Cancer
Patent
1985-03-18
1987-10-20
Richman, Barry S.
Chemistry: analytical and immunological testing
Cancer
422 61, 436 93, 436 71, 436 87, 436164, 436178, G01N 3348, G01N 3366, G01N 3392
Patent
active
047014180
ABSTRACT:
The amount of lipid bound sialic acid in a whole blood sample may be determined by a method, which may be automated, involving the following steps: adding to the sample a lower alkyl alcohol and deionized distilled water; mixing the resulting admixture; adding to it a mixture of lower alkyl chlorinated hydrocarbon and a lower alkyl alcohol; treating by mixing and centrifuging the mixture until a recoverable, substantially clear upper phase forms; recovering the upper phase and adding to it a protein-precipitating agent, preferably in admixture with an adsorbing material; mixing the resulting admixture; recovering the resulting precipitate, suspending the precipitate in distilled water and determining the amount of lipid bound sialic acid present. The presently preferred mixture for addition to the upper phase is a mixture of 75% by weight phosphotungstic acid and 25% by weight silica gel on a dry weight basis. By so determining the amount of lipid bound sialic acid present in a whole blood sample and comparing the amount with values obtained for subjects known to have cancer one may diagnose the presence of cancer in a subject. Alternatively, by determining the amount of lipid bound sialic acid present in whole blood samples obtained from a subject over a period of time and comparing each amount so determined with preceding amounts one may monitor the progression, remission or recurrence of cancer in a subject.
REFERENCES:
patent: 4115062 (1978-09-01), Moore et al.
patent: 4342567 (1982-08-01), Katopodis et al.
patent: 4541987 (1985-09-01), Guadagno
Kloppel et al., Proc. Nat'l Acad. Sci., U.S.A., vol. 74, No. 7, pp. 3011-3013, 1977.
Albouz et al., Chem. Abstracts, v. 92 (21135b) 1980.
Silver et al., Cancer Research, v. 39, pp. 5036-5042, 1979.
Delahunty C. M.
Dianon Systems, Inc.
Richman Barry S.
White John P.
LandOfFree
Method for determining lipid bound sialic acid in whole blood does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for determining lipid bound sialic acid in whole blood, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for determining lipid bound sialic acid in whole blood will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1612344