Method for determining lipid bound sialic acid in plasma or seru

Details Method for determining lipid bound sialic acid in plasma or seru Method for determining lipid bound sialic acid in plasma or seru

1993-11-05

1995-10-31

Redding, David A.

Chemistry: analytical and immunological testing

Heterocyclic carbon compound

Hetero-o

436 64, 436 71, 436129, 436178, 436813, 435810, 422 61, G01N 3348, G01N 3122

Patent

active

054628776

ABSTRACT:
The amount of lipid bound sialic acid in a blood plasma or serum sample may be determined by an improved method, which may be automated, involving the following steps to be performed simultaneously on the sample and a standard consisting of commercially available n-acetyl nueraminic acid (NANA); diluting with a buffer; mixing the diluted sample; adding a mixture of a chlorinated lower aklyl hydrocarbon and a lower alkyl alcohol; treating by mixing and centrifuging to yield a substantially clear upper phase; treating the upper phase with a color development reagent; mixing; boiling the admixture; cooling the admixture; and determining the amount of lipid bound sialic acid present in the sample by comparing the optical density of the sample to the optical density of the NANA.

REFERENCES:
patent: 4342567 (1982-08-01), Katopodis et al.
patent: 4748128 (1988-05-01), Katopodis
patent: 5045453 (1991-09-01), Katopodis
patent: 5296346 (1994-03-01), Katopodis

Affiliated with

Also associated with

Rating

Method for determining lipid bound sialic acid in plasma or seru does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for determining lipid bound sialic acid in plasma or seru, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for determining lipid bound sialic acid in plasma or seru will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1771834