Method for determining lipid bound sialic acid in plasma

Details Method for determining lipid bound sialic acid in plasma Method for determining lipid bound sialic acid in plasma

1990-11-19

1994-03-22

Housel, James C.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

435 18, 435 2, 436 63, 436 64, 436 71, 436 87, 436 93, 436129, 436178, 436813, G01N 3348, C12Q 100

Patent

active

052963462

ABSTRACT:
The amount of lipid bound sialic acid in a blood plasma or serum sample may be determined by an improved method which may be automated involving the following steps to be performed simultaneously on the sample and a known standard prepared from human or animal blood or tissue; diluting with distilled water; mixing; adding a mixture of a chlorinated lower aklyl alcohol; mixing, diluting with water and then treating by mixing further and centrifuging to yield a substantially clear upper phase; recovering the upper phase and adding to it a protein precipitating agent, mixing the resulting admixture; recovering the resulting precipitate, suspending the precipitate in a hydrolysis agent and determining the amount of lipid bound sialic acid present by comparing the optical density of the sample to the optical density of the standard.

REFERENCES:
patent: 4701418 (1987-10-01), Katopodis
patent: 4748128 (1988-05-01), Katopodis
patent: 4837144 (1989-06-01), Sugiyama
patent: 5045453 (1991-09-01), Katopodis

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