Chemistry: analytical and immunological testing – Cancer
Reexamination Certificate
2007-08-28
2007-08-28
Canella, Karen A. (Department: 1643)
Chemistry: analytical and immunological testing
Cancer
C436S071000, C436S813000, C436S815000, C530S419000, C530S420000, C530S424000, C530S424000
Reexamination Certificate
active
09802457
ABSTRACT:
The amount of lipid associated sialoprotein (LSP) in body fluids such as cerebrospinal fluid, peritoneal fluid, pleural fluid, bronchial washings, saliva and sputum samples, may be determined by a method which may be automated, involving the following steps to be performed on the sample: adding a mixture of a chlorinated lower alkyl alcohol; centrifuging to yield a substantially clear upper phase; recovering the upper phase and adding to it a protein precipitating agent; mixing the resulting admixture; recovering the resulting precipitate; washing the precipitate with saline solution; centrifuging to recover the precipitate; dissolving the precipitate in water; mixing; adding to the resulting mixture an hydrolysis agent; heating; and determining the amount of lipid associated sialoprotein present by determining the optical density of the sample.
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Bollo, Esq. Paul L.
Canella Karen A.
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