Method for determining .gamma.-glutamyltransferase activity and

Details Method for determining .gamma.-glutamyltransferase activity and Method for determining .gamma.-glutamyltransferase activity and

1984-08-27

1986-01-28

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving transferase

435810, C12Q 148

Patent

active

045671389

ABSTRACT:
A substrate solution comprising (a) a substrate selected from a group consisting of salts of L-.gamma.-glutamyl-p-nitroanilide and L-.gamma.-glutamyl-3-carboxy-p-nitroanilide; and (b) a polyol selected from a group consisting of alkyl polyols containing 2 to 10 carbon atoms and 2 to 10 hydroxy groups and polyethylene glycol having an average molecular weight of from about 200 to about 600.
Also, a kit for the determination of .gamma.-glutamyltransferase activity of the type comprising (a) a buffered medium comprising a buffer and a .gamma.-glutamyl residue acceptor; and (b) a substrate solution; the buffered medium and substrate solution being adapted such that preselected aliquots thereof can react in the presence of .gamma.-glutamyltransferase to produce an absorbing cleavage product. The kit is characterized in that the substrate solution is as described above.
In addition, a method for determining .gamma.-glutamyltransferase acitivity of the type comprising (a) contacting in any preselected order an aliquot of a buffered medium comprising a buffer and a .gamma.-glutamyl residue acceptor, an aliquot of a substrate solution, and an aliquot of a sample to be assayed to thereby form a cleavage product; and (b) measuring the absorbance of said cleavage product. The method is characterized in that the substrate solution is as described above.

REFERENCES:
patent: 3892631 (1975-07-01), Carroll
patent: 3979447 (1976-09-01), Bernt et al.
patent: 4372874 (1983-02-01), Modrovich

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