Method for determining endotoxin concentration

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or...

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435 34, G01N 2147, C12Q 100

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active

042218667

ABSTRACT:
Improvements in the sensitivity and reproducibility of nephelometric endotoxin determinations using endotoxin-activated enzyme from Limulus amebocyte lysate are achieved by contacting endotoxin-coagulated lysate with an ionic surface active suspending agent, generally a mixture of detergent and suspending agent. Instrumentation improvements further enhance results.

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patent: 4148869 (1979-04-01), Deaton
Wildfeuer et al.Applied Microbiology, 28(5), 867-871 (1974).
Solum, Thrombosis Research, 2, 55-70 (1973).
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Gaffin, Biorheology, 13, 273-280, (1976).
Levin et al., Throm. Diath. Haemorrh., 19, 186-197 (1968).
Solum, Throm. Diath. Haemorrh., 23, 170-181 (1970).
Young et al., J. Clin. Invest., 51, 1790-1797 (1972).

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