Method for determining endotoxin concentration

Details Method for determining endotoxin concentration Method for determining endotoxin concentration

1978-10-06

1980-09-09

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

435 34, G01N 2147, C12Q 100

Patent

active

042218659

ABSTRACT:
Improvements in the sensitivity and reproducibility of nephelometric endotoxin determinations using endotoxin-activated enzyme from Limulus amebocyte lysate are achieved by contacting endotoxin-coagulated lysate with an ionic surface active suspending agent, generally a mixture of detergent and suspending agent. Instrumentation improvements further enhance results.

REFERENCES:
patent: 3915805 (1975-10-01), Levin
patent: 4038029 (1977-07-01), Teller et al.
patent: 4148869 (1979-04-01), Deaton
Niven, Industrial Detergency, pp. 44-45 (1955).
Putnam, Advances in Protein Chemistry, 4, 79-122, (1948).
Solum, Throm. Diath. Haemorrh., 23, 170-181 (1970).
Young et al., J. Clin. Invest., 51, 1790-1797, (1972).
Wildefeuer et al., Applied Microbiology, 28 (5), 867-871 (1974).
Solum, Thrombosis Research, 2, 55-70 (1973).
Gaffin, Biorheology, 13, 273-280 (1976).
Levin et al., Throm. Diath. Haemorrh., 19, 186-197 (1968).

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