Data processing: measuring – calibrating – or testing – Measurement system in a specific environment – Chemical analysis
Reexamination Certificate
2007-06-19
2007-06-19
Sines, Brian (Department: 1743)
Data processing: measuring, calibrating, or testing
Measurement system in a specific environment
Chemical analysis
C422S050000, C422S068100, C422S081000, C422S082000, C422S105000, C436S043000, C436S063000, C436S180000, C702S001000, C702S022000
Reexamination Certificate
active
11482342
ABSTRACT:
The present invention is directed to a method for determining the molecular weight and diffusivity of a sample solute by providing a plus shaped microchannel network on a microfluidic chip, having at least four microchannels intersecting at a cross point; flowing a sample stream comprising a sample solute of unknown diffusivity and a blank stream from separate microchannels through the cross point and out to separate microchannels; creating a sample curve measuring the concentration of the sample solute that diffuses from the sample stream to the blank stream at the cross point while altering the flowrate of one of the blank stream or the sample stream; and determining a diffusion coefficient of the sample solute by extrapolating data from similar curves of at least two solutes having known molecular weights and/or diffusion coefficients created under similar conditions as those generated by the sample solute.
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Costin, et al., “Diffusion Coefficient Measurement in a Microfluidic Analyzer Using Dual-Beam Microscale-Refractive Index Gradient Detection—Application to on Chip Molecular Size Determination,” Journal of Chromatography A,, Sep. 27, 2003, pp. 77-91, vol. 1013, No. 1-2.
Caliper Life Sciences, Inc.
McKenna Donald R.
Peterson Ann C.
Sines Brian
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