Method for determining chondrocalcin

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435 79, 435 792, 435810, 436518, 436525, 436512, 436811, 436815, 53038885, 5303893, G01N 3353, G01N 33553, G01N 33543

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active

053745337

ABSTRACT:
A method for determining chondrocalcin through an immunoassay using an enzyme(s)-labeled mammalian chondrocalcin or enzyme(s)-labeled anti-chondrocalcin antibody which is characterized by that mammalian body fluid is used as a specimen and immunoreaction in a solution is utilized to determine the chondrocalcin of the mammalian, reagents and a kit therefor.
This determination can be utilized for diagnosis or the like for cartilage-relating diseases in mammals.

REFERENCES:
Choi, H. V., et al., J. Biol. Chem., vol. 258, No. 1, pp. 655-661 (1983).
E. Livne et al., "Age-related changes in the role of matrix vesicles in the Mandibular Condylar Cartilage" J. Anat.; vol. 150; pp. 61-74; 1987.
A. Hinek et al., "The Calculation of Cartilage Matrix in Chondrocyte Culture: Studies of the C-Propetule of Type II Collagen (Chondrocalcin)"; The Journal of Cell Biology; vol. 104; pp. 1435-1441; May 1987.
A. R. Poole; "Association of an Extracellular Protein (Chondrocalcin) with the Calcification of Cartilage in Endochondral Bone Formation" The Journal of Cell Biology; vol. 98: pp. 54-65: Jan. 1984.
Kuroki et al., "Two-Site `Simultaneous` Immunoassay with Monoclonal Antibodies for the Determination of Surfactant Apoproteins in Human Amniotic Fluid," Pediatric Research, vol. 19, No. 10, pp. 1017-1020 (1985).
Vogt, Jr. et al., "Quantitative Differences Among Various Proteins as Blocking Agents for ELISA Microtiter Plates," Journal of Immunological Methods, vol. 101, pp. 43-50 (1987).

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